The use of software in biological applications has given a new dimension to the field of bioinformatics. Many different programs for the design of primers are now available. Freeware software is available on the internetand many universities have established servers where a user can log on and perform free analyses of proteinsand nucleic acid sequences. There are number of simple stand-alone programs aswell as complex integrated networked versions of thecommercial software available. These software packagesmay be for complete DNA and protein analysis, secondary structure predictions, primer design, molecular modeling, development of cloning strategies, plasmid drawing or restriction enzyme analyses